Web25 Jul 2009 · Human hepatoma HepG2 cells (American Type Culture Collection, Manassas, VA) and Huh7 cells (kindly provided by Dr. Yumin Xu in Dr. Boris Yoffe’s laboratory, Baylor College of Medicine, Houston, TX) were cultured in MEM (InVitrogen, Carlsbad, CA) with 10% FBS, 1 mM sodium pyruvate and penicillin-streptomycin antibiotics (10 U/mL, 10 … Web21 Jun 2024 · Given these observations, we investigated how media composition and O 2 levels affect cellular energy metabolism and mitochondria network structure in MCF7, SaOS2, LNCaP, and Huh7 cells. Cells were cultured in physiologic (5%) or standard (18%) O 2 levels, and in physiologic (Plasmax) or standard cell culture media (DMEM).
Three-dimensional Huh7 cell culture system for the study of …
Web1 Nov 2024 · Huh7 cells were plated in 12 well plates at a density of 2 × 10 5 cells per well and cultured in supplemented DMEM media. All of the subsequent drug treatments were … WebHuh7 Cell Line Gene Knockout Most recent answer 24th Aug, 2024 Salah Adlat Mayo Clinic I am attaching here the puromycin kill curve protocol I am using for Huh7 and Hep3B cell lines if someone... georgetown white vanity
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WebCell line profile . Hep-G2 (ECACC catalogue no. 85011430) Cell line history . Hep-G2 is a human liver cancer cell line that was derived from the liver tissue of a year 15 WebHuh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly … Web12 Apr 2024 · In Huh7 ATG5-WT cells, ALIX puncta were induced and ALIX co-localized with lysosomes upon 30 min of LLOMe exposure, whereas in Huh7 ATG5-KO, this response was diminished ... cells were plated in 10cm dish in DMEM with 10 % FBS and infected with 500μL of virus-containing media overnight, and puromycin added for selection. christian fiedler berlin