Hoechst 33342 solution
NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … NettetSolubility Data for Hoechst 33342 Preparing Stock Solutions for Hoechst 33342 The following data is based on the product molecular weight 561.93. Batch specific molecular weights may vary from batch to batch …
Hoechst 33342 solution
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NettetHoechst33342 用于活细胞或固定细胞和组织的细胞核的特异性染色。 该染色剂通常与 5-溴-2'-脱氧尿苷 (BrdU) 标记结合使用，以区分凋亡细胞核的紧凑染色质，从而确定复制细胞并根据细胞 DNA 含量对细胞进行分类。 Hoechst 33342 和碘化丙啶通常一起用于对细胞凋亡和细胞周期分布各个阶段同时进行流式细胞和荧光成像分析。 For Research Use … Nettet18. jun. 2024 · Subsequently, the cells are incubated in a solution of 2 µM Hoechst 33342 or 3 µM DAPI in 20 mM Tris-HCl, pH 7, 150 mM NaCl for 30 minutes on a laboratory shaker (100 µl per well of a 96-well ...
NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, …
NettetThe Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the … Nettetsolution in water Store at 2–6°C Protect from light • • When stored as directed, product is stable for 6 months. Hoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from light • • When stored as directed, product is stable for at least 12 months. Hoechst 33342 ...
NettetMaterials and methods: Earle’s balanced salt solution (EBSS) was used to simulate glucose deprivation. MTT assay was used to analyze the cell survival rate, and Hoechst 33342 dye was used to detect the apoptosis in HeLa cells. Western blotting was used to detect the expression of ER stress and autophagy relative proteins.
NettetH342 -Cellstain- Hoechst 33342 solution. CAS No. 23491-52-3(Hoechst 33342:free base) Chemical name. ... Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively. Chemical Structure. how do you calculate rtyNettetBio-Rad's PureBlu™ Hoechst 33342 Dye is ideal for routine nuclear staining in fluorescence microscopy, cell imaging applications and cell cycle analysis in flow cytometry. It is available as a ready-to-reconstitute, highly pure powder form, with only one dilution step required to obtain a ready-to-use solution. Hoechst 33342 Binding how do you calculate rsd in excelNettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … how do you calculate schedule c incomeNettetIn 1978, another DNA-binding fluorochrome, Hoechst 33342, ... 5% FBS was used, and each well of a 24-well plate was coated with 200 µL of a 25 mg mL −1 Laminin–PBS solution. Prof. T. Kempf kindly provided rat ventricular cardiomyocytes. A detailed protocol for isolation and cultivation is described here. pho noodle companyNettetProduct number: 910-3015 Size: 1 ml Storage: 2 – 7°C Solution 15 – Hoechst 33342 is a staining reagent containing Hoechst 33342, which stains the nuclei of living, fixed and permeabilized cells. Solution 15 – Hoechst 33342 is a cell permeable dye that preferably stains doublestranded DNA. pho noodle expressNettetbisBenzimide H 33342 trihydrochloride. Synonym (s): 2′- (4-Ethoxyphenyl)-5- (4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, … pho noodle gilroyNettet27. mar. 2015 · DNA condensation in cells was stained with Hoechst 33342 solution and visualized using a fluorescent microscope (magnification 100×). BSA, bovine serum albumin; PA, palmitate; Vc, Vitamin C. Figure 3. Protective effect of hispidin on palmitate-induced oxidative stress in C2C12 myotubes. how do you calculate semi monthly salary